In this webinar, Dr. John Gildea & Dr. Martin Katz, discuss five root causes of autism with physicians. Slides can be downloaded here.
They discuss a Johns Hopkins paper looking at sulforaphane's impact on this population which can be found by clicking here.
Highlights
2:50 – Reactive Oxygen Species (ROS)
6:30 – Mitochondrial Dysfunction
17:57 – Inflammation
23:21 – Gut Health
28:25 – Sulforaphane reduces symptoms of Autism: clincal trial overview
34:10 – Sulforaphane vs glucoraphanin (aka sulforaphane glucosinolate)
46:30 – Euric Acid (mistakenly called eurcin in the webinar) in broccoli
49:30 – Clinical testimony on sulforaphane’s impact on Autistic children from Dr. David MacDonald*
*These statements (in the webinar) have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease.
**Sale ends at 11:59 PST, 2/11/19. Offer does not apply to previous orders. BrocElite Professional is sold only to clinicians at wholesale.
Transcript
David Roberts:
All right, welcome everyone. Welcome to our webinar. Tonight we'll be talking about the root causes of autism and actually five of the 10 answers from science. We're going to do five tonight and five next week, just from a time standpoint, it's a quite a bit to cover.
David Roberts:
My name is David Roberts and with me tonight are Dr. John Gildea and Dr. Martin Katz. Dr. Gildea is a professor at the University of Virginia and heads up a core lab there. Has lots of research projects that he's done, and many peer reviewed journal articles. He was instrumental and still is instrumental in the research behind the gut supplement, Restore, which some of you may know, but he also was the one that stabilized the sulforaphane molecule in broccoli that helped us bring it to market. So welcome, John.
David Roberts:
And also Dr. Martin Katz is a board certified physician in Charlottesville, Virginia. His passion is to meet with patients and to talk to them about how to improve their health through nutrition and exercise. And so welcome to both of you.
David Roberts:
Tonight we're going to be talking about ROS and then intimately related to that mitochondrial dysfunction and then detoxification, inflammation and gut health in that order. And we'll be talking next week about sleep, depression, epilepsy, infection and anxiety as it relates to autism. So the format will be talking about the problem, what it affects and why children with autism have that problem. And then looking at the literature and some holistic solutions and tips, and the answers really that are from the literature. So I think we all know autism is a significant problem and a growing problem. Three and a half million Americans and that's one out of every 37 boys, one out of every 151 girls and that prevalence is just a shooting up. And so without further ado, Dr. Gildea,
Dr. Gildea:
Yeah, it's good to be here. First topic of discussion tonight is a reactive oxygen species. It's a word that's thrown around a lot and hopefully we can shed light on the basic problems with having too high of reactive oxygen species. So in general, the problem is that stress or other influences that we'll talk about later, can increase ROS. And what's found is that ROS is higher in autistic kids and a lot of different tissues and contexts. And what does it do? So a reactive oxygen species in general is thought of as an unpaired electron and obviously in an oxygen containing molecule intimately tied with mitochondria. But these free radicals are able to interact with proteins, lipids, DNA, RNA, and they basically just create damage just kind of like a fire. And so one of the big connections with autistic kids is that they seem to be more sensitive to production of reactive oxygen species, and some of the SNIPS, single nucleotide polymorphisms, that are associated with autism point towards this.
Dr. Gildea:
And also the fact that they don't handle environmental toxins as well. They're not getting rid of environmental toxins as well. So in the case of reactive oxygen species in these kids, the connection to sulforaphane is that the molecule NRF2 is a molecule that is kind of the gatekeeper for handling reactive oxygen species. When reactive oxygen species are high, NRF2 is increased and turns on most of the enzymes that are called antioxidant response element driven genes. And the way to combat this is when you get NRF2 activated, it dissociates with keap1 and then NRF2 is stabilized. And so it avoids being degraded and then travels to the nucleus and turns on a bunch of genes that are themselves enzymes that get rid of reactive oxygen species.
Dr. Gildea:
So as opposed to taking antioxidants, everyone knows that foods that are good for you tend to have more antioxidants in them, but this system when it's turned on, it turns on enzymes that continuously turn off these reactive oxygen species in all the different compartments and the membranes. And because it's enzymatic, they keep turning them off over and over again. So it's a very potent way to turn off antioxidants. Two good examples of this is sulforaphane to turn off and turn on NRF2 and curcumin is another one, slightly less potent, but also good at activating NRF2.
David Roberts:
All right, moving on.
Martin:
So next, this is Dr. Gatson. Thank you so much for coming or signing up. This is obviously an incredibly important talk both clinically for us and for some of us, even maybe personally. As a parent, we obviously want to have healthy children who are functional and doing well. And when we have a child who maybe presents with less function, it changes things. And so we obviously want our environment to be healthy, we want our patients to be healthy and certainly want our loved ones to be healthy. I'm blessed to be able to talk about this, blessed to be able to bring a molecule like sulforaphane to the market.
Martin:
Speaking about mitochondrial dysfunction and disease... Mitochondria is obviously just an incredibly important part of the cell and what we realized about the mitochondria going through biology as younger kids is it's this powerhouse of the cell. And then hopefully later on, we realized that it's not just providing us with energy, but also providing us with cellular speak and communication. And so it was an integral part of the cell and a very important part. And unfortunately, when there's dysfunction cellular communication goes awry. And obviously we see autistic kids with significant communication problems, which is, I guess, a bigger manifestation, but again, this mitochondrial dysfunction and oxygen leak... You'll have problems with energy. These kids won't be as energetic possibly, but also their cells are not communicating.
Martin:
So the exact way to look at this in autistic kids is unclear. But again this whole role that the mitochondria is playing with oxygen as a receptor of these electrons and this whole ROS system as communication of this is altered, there's going to be a significant dysfunction in how the cells are doing in an unhealthy state. So some of the ways we look at, or I look at it in my practice to help my patients with mitochondrial dysfunction, obviously trying to get your kids to exercise. We know that exercise has a phenomenal influence on mitochondria and mitochondrial health. So we see a lot of mitophagy and ensuing mitogenesis with exercising. And we're getting part of these mitochondria that are dysfunctional, we're recycling the protein, and then building healthy mitochondria.
Martin:
So obviously any effort we can have to get these kids playing outside or getting some form of exercise, a lot of autistic kids love music. Getting them to dance around and just enjoy the experience is good. There is a molecule that tends to turn on fat metabolism and oxidative stress within the cell. And that's a molecule called the SIA. That's been shown to boost energy through fat metabolism. And sulforaphane has been shown in cell cultures to lead not only to mitophagy and getting rid of these unhealthy mitochondria, but also in stimulating mitogenesis. So obviously with that, you will have increased in NAD and improvement in energy production. So a lot of good things happening there.
David Roberts:
So real quick, Kathleen Jones, you send a question in about the slides. Can everyone see the slides? Can you guys type in whether you can see the slides or not?....
David Roberts:
What's that? Click that. All right. Thank you man of the hour. Okay.
David Roberts:
How about now?
David Roberts:
Cliff Thompson said, no. Cliff, can you say whether you can see the slides now... Anybody? But I think that may have resolved it. So let us move on.
David Roberts:
So mitochondrial dysfunction, and then now we're going to talk about detoxification.
Dr. Gildea:
Yes. So the General idea behind detoxification's very similar to reactive oxygen species, and that it's all the genes that are part of the detoxification pathway, or almost all of them, are also regulated by NRF2, this protein. And it's generally understood that children with autism have problems in the detoxification pathway. So the one measure of detoxification that's been done over and over in autistic kids is their glutathione status. Glutathione is one of a large number of enzymes that are part of the detox pathway. And you can take cells from an autistic child and measure the amount of reduced glutathione that that child has; GSS to GSSG ratio. And it's actually close to diagnostic, a large number of kids that have problems with glutathione production. So because of that, these kids will react differently to the same amount of toxin that's not a problem with other kids that are around them or other adults that were experiencing the same environmental insults.
Dr. Gildea:
It's not good for anybody, but these kids are the canaries in the coal mine. So they are the ones that we first see these toxins in our environment on. There are a lot of factors in that can be as simple as fire retardant, as prevalent as mothers living close to fields where they're spraying, organophosphate, pesticides. There're some studies showing that truly these kids are more sensitive to environmental toxins.
Dr. Gildea:
So ways to actually go about treating this disease state is by unblocking this pathway. Sulforaphane is a really great way to do. It's been shown many different times to induce phase two detoxification. It's probably the strongest natural Phase 2 detoxfier and should be extremely helpful for these kids that are more sensitive to toxins in order to get rid of those toxins.
Dr. Gildea:
A couple of ways that have been shown to get rid of, especially heavy metals is cilantro and cell decimated chlorella or broken cell chlorella. But those are basic strategies for addressing this issue, but the detoxification and trying to get a number of environmental toxins out of your system, simplest is eating organic and then looking for potential toxins in the household. There could be a whole webinar just on trying to find environmental toxins and getting rid of them. So, but yes that's a real quick summary.
David Roberts:
Yes. I want to encourage folks if you are on the webinar portion to go ahead and type in questions for our Q and A time. I think you will have the phone option. There are some of you just on the phone. If you have a question, I believe there's a way to raise your hand through the phone. We can call you verbally.
David Roberts:
So just on that detox, what would you say is sort of...?
PART 1 OF 4 ENDS [00:16:04]
David Roberts:
Just on that detox, what would you say is sort of an example of a toxin or sort of one that most autistic children are having to deal with? Do you have one off-hand that you, comes to mind?
Dr. Gildea:
I think it's glyphosate for sure is one of the biggest ones. It's a pesticide or herbicide that is so prevalent in our system that there's really, very difficult to get it out of your system outside of eating organic.
David Roberts:
We have this graph. You want to talk about this graph.
Dr. Gildea:
Yeah, basically, it's just showing that the rates of glyphosate usage in the United States gone up incredibly rapidly, and it's just a pervasive environmental toxin that is affecting a lot of the pathways that we know are involved in... that are issues in autistic kids. So just going organic would be a great way to eliminate that pervasive toxic.
David Roberts:
Yeah. And this is showing correlation. We knew correlation doesn't necessarily mean causation. However, there have been some studies linking the two, I believe.
Martin:
Now I think there's also some studies on decreasing kids' exposure to gluten and casein, showing some improvements in autism that would go along with that.
Dr. Gildea:
Right.
David Roberts:
Right. Inflammation.
Martin:
Inflammation's pretty much the whole reason I continue to practice medicine, talking to my patients across the spectrum about inflammation, and now there's a more well-known term of inflammaging. We're not going to address inflammaging today, but we're going to talk about inflammation as it relates to kids with autism as this, again, presents as a major problem. Inflammation can come from numerous different avenues. Our biggest concern, again, in kids with autism, it's been shown that kids with autism have a gut inflammatory problem. If you have inflammation along the gut lining, you're going to have significant problems with absorption.
Martin:
We all, at this point, probably know that most of our immune system sits along the gut lining, known as the GALT, gut-associated lymphatic tissue. This tissue is obviously monitoring what's coming in, as it should. This is our first-pass look at our environment, and so we need to be pretty keyed into things that are going to hurt us. And so, obviously, if there's a lot of inflammation around the gut, we would expect that those T lymphocyte cells don't just hang out around the gut. They're going to migrate and create problems. And unfortunately, in our autistic kids, it seems like the brain is extremely sensitive to this and is the end-causal result. And so, it's affecting their brain, and with that comes problems with brain development and brain function.
Martin:
And so, we see these exacerbations in their early life as they're going through these developments, which makes a lot of sense. Unfortunately, if you are a parent with a child, any child really, but certainly with a child with autism, their diets are pretty selective. They definitely seem to trend towards higher carbohydrate foods, higher sugar foods, oftentimes quite hard to encourage them outside of a small spectrum of foods. Unfortunately, this leads to further problems with dysbiosis which, as we all know that, the bacteria is one of the things that supports the gut, the gut lining. And if that thing becomes a problem, we're going to see more inflammation on the other side as this GALT system is seeing things that it shouldn't be seeing.
Martin:
So this is a huge part of helping these kids is really understanding inflammation and certainly understanding inflammation at the gut level. There's lots of things that can cause inflammation. Again, we just spoke about toxins, certainly infections, both when they're in the womb and shortly thereafter. But looking at the gut and really helping gut inflammation is key, so again, getting them on some type of improved diet where you're slowly introducing foods along with the foods that they enjoy. Maybe using a blender quite readily, using crock pots, using soups, anything that you can do to get these kids out of the small amount of foods that they're doing. Obviously, we know fiber foods, then have probiotic usage and then prebiotics... sorry, prebiotic, then probiotic. So certainly improving their diet is going to be essential.
Martin:
And then certainly product like sulforaphane and curcumin, addresses inflammation. In the studies, we know that these are two of the strongest inhibitors of NF-kappa B, and all the drug companies are definitely going after NF-kappa B to limit inflammation. So, if you can target this molecule, the interleukins, which sulforaphane does a very good job of decreasing certain cytokines, which are inflammatory, decreasing NF-kappa B, you're going to make a fairly good impact on inflammation. Again, it's sort of seems crazy that we keep talking about sulforaphane in all these different ways, but this molecule is so incredibly effective at the genomic level to turn on this 200 genes of this antioxidant response element and has such a pleiotropic response, such a across-the-board response.
Martin:
And it's really exciting for us to being able to stabilize this molecule and deliver a known dosage to the patient where if their gut's unhealthy and you're giving them precursor molecules... And we'll talk about exactly what precursor molecules and what sulforaphane is in a second. But if you're giving this unhealthy gut a precursor molecule, the chances of them getting sulforaphane are incredibly small, 0%, certainly below 10%. So having a molecule that's stable and known dosages is very important to decrease inflammation.
Dr. Gildea:
So Martin already talked a little bit about gut health, how centrally important it is in inflammation.
Martin:
We can talk a lot about gut health.
Dr. Gildea:
Yeah. Absolutely, super important, and it impacts every aspect of-
David Roberts:
just a reminder, text in questions, because we're readily approaching our Q&A time.
Dr. Gildea:
Yeah. For sure, the gut and brain is linked and that probably things that happen in the gutter are affecting brain leakiness. A lot of the seminal work that was done at Hopkins showing that it's autoimmune and the mom to a child that's growing inside womb has to do with autoimmunity, why mom that has one autistic kid is more likely to have a second autistic kid. And that these autoimmune antibodies are found to be to small number of brain proteins. So this linked from gut health to brain health is a really important one.
Dr. Gildea:
The initial phases of intestinal dysbiosis is you get the bacteria not growing in the proper ratios are really important in how you're metabolizing sugar, when you’re getting sugar penetrating further into your gut and feeding the bad bacteria and making a bad cycle going on there and blocking that leaky gut so that those food particles are not actually being presented to the gut. Like Martin was talking about, this is a really key component to stopping this cycle. So a lot of naturopath physicians that are treating autistic kids have said over and over that you really have to address the gut health first before you can address some of the other ones.
Dr. Gildea:
Seems to be that because sulforaphane is been shown to affect kids' single-molecule that sulforaphane must be addressing multiple issues, including tightening tight junctions in the intestines, decreasing inflammation, doing a lot of things all at one time. Martin also mentioned earlier about casein, different things that are possible inflammatory molecules to the gut. So having a properly functioning digestive tract, digesting food properly is the goal. Sometimes you have to back off and do a very special diet at first. One really effective one is special carbohydrate diet, and that's been ranked on things that work for autistic kids very high. Gluten is certainly part of that, and trying a special diet, including diets where you're trying to eliminate foods that kids have reactions to, are a really important part of the puzzle.
Dr. Gildea:
But we like to say that Restore, another supplement that works really well for gut healing. Sulforaphane also has gut-healing capabilities as well as curcumin. One part about curcumin in that it's not very well-absorbed, actually helps in this area that more curcumin goes to the lining of the intestines, small and large intestines and can be an anti-inflammatory, specifically in the intestinal lining, which is really good. Quercetin is another molecule that has been shown to tighten junction. So there's a number of things to do in addition to the generally eat better, less pesticides. But this general strategy of getting gut health intact is a super important and central feature.
Martin:
I also read recently that sulforaphane seems to turn on GLP-2, helping the mucosal lining, a gut lining to be healthier, which was exciting.
David Roberts:
Right.
Martin:
So, we're going to talk about a clinical study done by folks up at Johns Hopkins. These are the guys that have started the Cullman Chemoprotection Institute. This is where Paul Talalay and Yuesheng Zhang found sulforaphane back in 1992 and realized that this was a very important molecule. And since that time, Jed Fahey has taken this molecule and looked at it, and we believe he's quite interested in this molecule with regard to autism and will be coming out with some studies with sulforaphane in autism.
Martin:
This is a study he had done previously, very exciting study on a population that can be quite difficult to treat, kids are a little bit older, 13 to 17 years of age. We'll bring that slide up in a second. But the study gave these kids sulforaphane at a specific dose. Do you mind going back to the previous slide? And they gave it to them for 18 weeks, and if you look at the slide, they showed significant changes in aberrant behavior, social interaction and also verbal scores up to 18 weeks. And you can see the kids getting better over an 18-week period. That's the blue line. But they called the sulforaphane after 18 weeks, and you can see that these children returned back to baseline fairly quickly.
Martin:
The good news is that sulforaphane is available for all your patients quite readily. If you want to be sprouting, if some of your families can't afford a supplement or aren't in the supplement market, sulforaphane is readily available... not so much in broccoli. You have to eat a ridiculous amount of broccoli a day, about nine pounds of broccoli a day. But if your families are interested in sprouting, you only need to eat about three ounces of broccoli sprout a day, and you can put that in salads.
Martin:
Again, most of your autistic kids aren't going to be eating salads, but if you blend it and then add it to certain things that they really like. And I wouldn't start at three ounces a day. I'd start quite slowly because they'll be on to you fast at three ounces a day. But start slowly and gradually over a period of time, add sprouts. The other thing you can do is get our liquid. We have a sulforaphane liquid, and you can add that to smoothies or soups or different things, not at temperature, and slowly increase the amount of sulforaphane. Not many kids are going to be taking the capsule, autistic kids are going to be taking the capsule. So this would be more liquid for them.
Martin:
Again, these kids were a more challenging age group, young men aged 13 to 27, and they showed significant improvement. There's this follow-up study where they called these kids or these parents and followed up with them after and they continued to show improvement, continued to show a response to sulforaphane in most, significant amount of these families that continued taking the sulforaphane. So a real plug for this molecule, which again, because of its effect on the genome, which again is one thing that doesn't change how we affect the changes, how we do across the board. Again, this speaks to the fact that autism, so many possible causes in this-
PART 2 OF 4 ENDS [00:32:04]
Martin:
... to the fact that autism, so many possible causes and this molecule, again, addresses a lot of it as to why we would see this incredible improvement. Great. Moving on. John do you want to ...
David Roberts:
I mean, one question that's come in is just the idea of these statistics apparently that are ... Those are significant, especially in this age group. Are there other compounds that have provided, in the clinical trial setting, improvements like this? Or near? Or even any? What is the lay of the land?
Martin:
Yeah, I think this was a double blind placebo study. It was a little bit easier. The kids we were provided. There are studies where parents undergo gluten-free and casein-free, and they undergo this massive effort to change. And so they see a big change. But a lot of those studies have been questioned how much was it a placebo effect, where the parents wanted to see the change because they were doing so much. And so some of those are being questioned. I'm not familiar with any other studies showing this kind of dramatic improvement in an adult placebo-controlled study. Obviously we'd like to see ongoing studies and bigger studies and hopefully again, Jed Fahey, hopefully we can be involved as well in getting this study on a bigger platform.
David Roberts:
Great. And just a reminder to everyone listening as you're online, please type in questions. So for Q&A time, as well as if you're on a phone, you can signal to raise your hand. All right.
Dr. Gildea:
Yeah. Just to talk quickly about how sulforaphane is made in general is there are precursor molecules. Glucoraphanin is the precursor molecule that's found highest in broccoli. And this is converted through an enzyme called myrosinase, in order to convert to sulforaphane. The act of making sulforaphane happens when you chew the broccoli and release the enzyme. Unfortunately, there's another enzyme that's also activated that inactivates sulforaphane. So most of the products on the market right now are the precursor molecule, glucoraphanin. And the better of those molecules also contain the enzyme myrosinase. BrocElite is fully formed sulforaphane itself, and so you don't have to guess at whether you are going to be a good converter of precursor or not. So you just get delivered the actual dose that would be very similar to the study that was done.
David Roberts:
Are there specific bacteria? What are the bacteria that actually do the conversion? Are there specific ones or are there families of them?
Dr. Gildea:
Yeah. In general the bacteria species that are able to convert glucoraphanin to sulforaphane are the same classes that are generally considered as healthy groups, if the bifido and bacteriodities, the good ones, and the ratio of firmicutes to bacteriodities, that ratio would be a decent predictor. But I think that's a very active area of study right now, which ones are actually capable.
Martin:
Yeah. This is important to understand this myrosinase is very sensitive to temperature. And so when you over cook your broccoli, you're certainly losing your myrosinase. If you're taking those broccoli sprouts and heating them, you're unfortunately going to inactivate myrosinase. You may get somewhat lucky in the gut level and have these healthy bacteria, but again, in the population we're talking about, this is not always the case. So we really need to have that myrosinase around. The glucoraphanin's fairly stable. Sulforaphane, once you get it to the cell or into the body's quite stable. But myrosinase is not so much and very, very much temperature inactivated at certain temperatures.
David Roberts:
And then there are studies or companies coming out with, or at least trying to stabilize the myrosinase. You guys hear about this? What are your thoughts on that?
Dr. Gildea:
There certainly is myrosinase in some products. Enzymes are kind of notorious for being easily inactivated. It's shape of a protein is difficult to keep active, but there are ... Rhonda Patrick's, one of her latest releases is saying that when you're eating broccoli or broccoli sprouts is to add mustard powder as well. So there are ways to increase your ability to convert. But I think it's not going to get up to the 100% of an actual sulforaphane product, so.
David Roberts:
Yeah, I listened to ... Actually, she said that a couple of times. And so I bought a big container of mustard powder that I think I used once. And it changed the taste of the dish so significantly.
Martin:
Michael Greger speaks of this, mustard seeds and mustard greens.
David Roberts:
Yeah. Mustard greens.
Dr. Gildea:
That must be a stable version of the myrosinase, or more stable.
Martin:
Radishes. Mm-hmm (affirmative).
David Roberts:
So a question in from Christina, the sulforaphane affects sulfur-sensitive people. And if it does, what can be done? Supplement with molybdenum?
Martin:
Molybdenum.
David Roberts:
Yeah.
Martin:
Yeah. You want to address that one?
David Roberts:
There's certainly sulfur in sulforaphane. The person who is sulfur sensitive would be sensitive to this sulforaphane. I don't really know. So sulfur itself is one of the most abundant minerals in the body, behind calcium. So I think the person who is sulfur sensitive is probably sensitive to foods that have really high content in it. And sulforaphane in our product is milligram amounts, so relatively small. So, but yeah.
Martin:
I would say-
David Roberts:
-for sure.
Martin:
I would totally agree with John. This sulfur molecule is not something that's unique to it. So you're going to see it all over the body. But if you have somebody who seems to be sulfur sensitive, or a slow metabolizer, what I tell my patients is do it slowly. Open a capsule. There's no problem with opening the capsule, adding a small amount to your food, seeing how you react to it. If you react to that, add a lesser amount. If you don't react, start adding more, but slowly. So I instruct my patients just that I shouldn't be telling them what they should be doing. They should be listening to their body to figure out what they should be doing. But ways that I guide them through that is just adding smaller amounts, if they're worried about buying this product and if not, and it being not good.
Martin:
Certainly you can go to Whole Foods and buy a little package, I think it's about a six ounce package of broccoli sprouts already made, if you don't want to get into the whole process yourself. I think it's a four ounce or six ounce package, four ounce package of ... We got confirmation on four, somebody taking four, four ounce package of broccoli sprouts. And they can slowly do that and see how they do with the broccoli sprouts. So that'd be another way before they commit to getting this product or before they commit to sprouting broccoli seed.
David Roberts:
Great. I don't have much connection with molybdenum. I think I might have to look that up.
Martin:
It's on the periodic table I know.
Dr. Gildea:
I do want to go back to the sulfuricin breakdown and how it's created. So this is confusing. Yeah. And my background is in the sciences and it was confusing to me. So when my wife was diagnosed with breast cancer in 2012, we tested her and found that sulforaphane was very important for her healing. But when we went out and bought sulforaphane glucosinolate, and I think we bought over a course of years, thousands of dollars’ worth of that, of a professional grade, and thinking that was sulforaphane, but I mean, but it's not. What is it?
Martin:
Well, sulforaphane glucosinolate is glucoraphanin. So it's a great marketing ploy to get in the same ballpark as sulforaphane, or get people's minds in the same ballpark as sulforaphane. But it is not the same molecule, it is actually the precursor molecule, glucoraphanin. So you absolutely need to have either the healthy bacteria around to convert it to sulforaphane, or you need to have myrosinase along with it to convert it to sulforaphane.
Martin:
But again, in the studies that were done by again, Jed Fahey up at Johns Hopkins, the absorption of these supplements is very low, oftentimes below 10%. And so if you're dealing with an unhealthy population, you want to be fairly sure about what you're getting, again, because it's so effective at the cellular level. You don't want to be guessing at this. You want to know what you're getting. And so having a, having a stabilized sulforaphane molecule is a real boost to that. Yeah.
David Roberts:
Yeah. And along those lines, Doctor Gildea, you've tested some of the precursor molecules, supplements. What have you found, not naming names?
Dr. Gildea:
Yeah, no names. So the precursor obviously doesn't have any effect on pathways that we're talking about. And because we know how to make sulforaphane, we would take some of the precursor products out there and put them in the ratios and in the right environment that we know they should make sulforaphane. But it was really ... They were not making a decent percentage of the sulforaphane, given the ... We know all the co-factors and the amount of the volume [inaudible 00:43:58] expended to try and mimic digestion. And they were definitely underperforming ones that we got.
David Roberts:
Okay. Another question coming in. Again, please feel free to type in questions for those who are just arriving. Have you done any clinical trials on BrocEite?
Dr. Gildea:
We do have a clinical trial on the books, and we have looked at some readouts of that. So normal dose, actually on the low range, and seeing effects on inflammatory markers in urine is statistically significant. So yeah, we have tested it on people.
David Roberts:
And what did you find?
Dr. Gildea:
Yeah, there was a significant reduction in inflammatory markers that are secreted in urine. So looking forward to getting that shaped up and do a paper on that. The molecule that we were looking at is interleukin 6 which is a known downstream molecule from NF-κB and interestingly sulforaphane affects NF-κB. And so, with the normal dose that we have in our capsules affected that directly.
David Roberts:
What was the amount that it aided or decreased the ...
Dr. Gildea:
It was in the 30% range.
David Roberts:
With how much?
Dr. Gildea:
Four milligrams.
David Roberts:
Okay. One dose?
Dr. Gildea:
It was two days. We did a placebo and then two days of taking sulforaphane and measuring the urine.
David Roberts:
Great. All right. Other questions.
Dr. Gildea:
I can bring up questions that I have.
David Roberts:
I'm sure. Why don't you do that.
Dr. Gildea:
That I had to look up.
David Roberts:
It's not a problem.
Martin:
Yes.
Dr. Gildea:
I was having a good time. One of the things that comes up in a lot of blogs that I've seen on sulforaphane or in broccoli in general, is erucin, the oil that is in broccoli seeds.
Martin:
Can you spell that for our audience?
Dr. Gildea:
E-R-U-C-I-N. And it's very interesting looking back at it, just to cut to the chase is canola oil. The original name was ... I forget the original name of it. But the canola oil comes from rapeseed and rapeseed was not good for consumption because it had too much erucin in it. And so they made a genetically modified seed so that it produces only 3% of the original seed. And so there's many people that are consuming upwards of 30 mils, two tablespoons a day. That will be easy dose, that's on a salad or something, of canola oil. And that amount of erusic acid that's in that would vastly exceed, by more than 10 fold, the amount that's in broccoli seeds, that would be in a common product like ours. So it's not an.
Martin:
[inaudible 00:48:03]
PART 3 OF 4 ENDS [00:48:04]
Dr. Gildea:
So, it's not an issue.
Martin:
Right. Right.
Dr. Gildea:
It's so, so low. And there-
Martin:
Weren't there some benefits as well to this?
Dr. Gildea:
The oil?
Martin:
Yeah.
Dr. Gildea:
Yeah. There's a broccoli seed oil on the market right now that's used for skin. Obviously they're using purified oil and putting it on their skin, and that amount of acid would be getting into you as well through the skin, and there hasn't been problems as far as I know.
David Roberts:
Great. We have a local clinician in town, David McDonald, who I'm going to try to patch in here, who has used sulforaphane on three children with autism. I think he's on the line, but I'll actually call him in here. Let's see if he picks up. Dr. McDonald, hey.
David McDonald:
Yeah.
David Roberts:
David Roberts here. So thanks for joining us, Dr. McDonald. So we have some clinicians on the line, or on the webinar. I just wanted you to share with them what you have seen as you've implemented using sulforaphane on autistic children. Maybe just pick one, and tell us about what you've seen.
David McDonald:
Well, I'd like to pick one. But if possible, I'd like to pick three or four. But let me just tell you, I just left my clinic two hours ago and I had a woman bring her son in who is on the autistic Asperger's spectrum. She brought him in because he's biting himself, and long story to that. But bottom line is, sometimes you get a serotonin release or it's along the lines of cutting. When they're stressed people will cut or do things, and this young man has been biting get some relief. But recently was started on BrocElite, and started changing his mathematics skills dramatically. In fact, he couldn't subtract two weeks ago, and he's now subtracting. His short-term memory has dramatically improved. His aberrant behavior, even though he's biting himself, but in the context of his aberrant behavior has significantly improved.
David McDonald:
So, I was really encouraged by the response that I've seen in this family. They have three or four children with autism, Asperger's, and all of them have noticed an improvement. The most remarkable thing has been one of the siblings is now developing speech, and they've only been on BrocElite less than a month. Maybe six weeks at the most. So in this one family, a mother of three children with Asperger's autism, I've seen significant improvement.
Dr. Gildea:
How's how's the mom feeling about that?
David McDonald:
Mom is ecstatic. She had tears in her eyes. Not to get too deep into the weeds, but there's a lot of marriage stress because of the challenges they face. So just the fact that her children have improved this much, she had tears in her eyes at the progress. She was thrilled today, especially with the mathematics skills. One thing I regret is, I wish I would have started out with a baseline autistic skills or markers, like the aberrant behavioral skills, or ... There are certain markers, and I wish I would've started so I've got more of a definitive clinical baseline, so that I could share that. But just mathematics skills alone and speech development are two of the things have been significantly improved.
David Roberts:
Those were ... At least the speech was in that clinical trial that you talked about, Dr. Katz. That was shown in the clinical trial.
Martin:
Yeah. Speech, certainly aberrant behavior, verbal communication, and social responsiveness that were all positively affected. So what Dr. McDonald is seeing in his clinical practice makes sense. I actually left the office three hours ago, and glad I beat you out by an hour there, Dr. McDonald. But I just met with a lady who has an autistic child, and to try and get extra money, she was having other kids that she would sit through the day. But unfortunately, her autistic son was showing worsening aberrant behavior and being threatening to the kids that are coming in, and she had to stop doing that. Since the child has been on BrocElite, he has shown tremendous improvement. She's now able to have other children come over and look after them again, which she's delighted. It's very helpful for her.
David Roberts:
Yeah. So for you, Dr. McCallum, and you, Dr. Katz, what are we talking about as far as how much sulforaphane are these children receiving?
Martin:
I'll let Dr. McDonald answer that.
David McDonald:
Yeah, so I will. I mean, I've only used this for two months and I'm seeing dramatic results. If you want any more case stories, let me just give you a brief one. This young boy came to me Saturday, last Saturday, because his mom, single mom, three children. He's on the autistic Asperger's spectrum. So I started on Saturday, started him on BrocElite Saturday. The report as of today, the mom said, "I can't believe this, but my son is already better on the bus." He was having a lot of challenges interacting with other kids on the bus. Significantly improved. He's in a swimming program, and last week, just last week, very dysfunctional, not obeying, not listening to mom. Today, everything is different.
David McDonald:
So, when you look at your ... I was listening to your clinical trials, how you saw the inflammatory markers change within 48 hours. I can tell you from my experience, it's only [inaudible 00:54:43], but this kid is completely different. I called his swimming coach and asked, "Is this really true?" And his swimming coach said, "I don't know what you did with him, but it's dramatically different just since last week."
David McDonald:
And then I have another family that the mom was having to wrestle her son to the ground. He's 10 years old. She literally had to wrestle him to the ground. So, go to the dosing question. I started out with just one a day with this 10-year-old boy, and within two or three weeks he was dramatically improved. So much so that she brought her other children in at the same dose of one a day, and so much so that three of her children who were previously very dysfunctional sat in my waiting room without their mother. They obeyed while I saw their mother, and their mother had tears. She said, "I can't believe I'm in your office by myself, and my children are out in your waiting room, and they're behaving."
David McDonald:
So to go to the point of dosing, all of the children that I've seen that are less than 13, 14, I put them on one a day. I have autistic Asperger's kids that are actually very tall. The family's tall, and they're about 6'2 to 6'3, and they weigh anywhere from 150 to 180 pounds, and I'm putting them on twice a day. So, that's the dosing that I'm doing. Kind of shooting from the hip, because they don't really know what dose is needed. But that's where I started. So 10 or less, one a day, depending on their height and weight. I don't know if there's a height/weight factor in this, but the older adult-size children I'm doing twice a day.
David Roberts:
Okay. So, that's five milligrams in one capsule. What is the phased clinical trial? What did they do? I know he likes to use millimole, so it's nobody. I always forget the conversion, but what does he use? Anybody? Do you know?
Dr. Gildea:
Sure. I read the paper recently. He often does 50 or 170 micromoles.
David Roberts:
So, 50 is 8 or 9 ...
Dr. Gildea:
8.8. Yeah.
David Roberts:
Okay. It doesn't say there. We'll have that for next week. Great. Well, Dr. McDonald, thank you. We have our part two next week, so we'll be in touch with you over the course of the week in case you have additional-
Martin:
Just real quick on that. As far as dosing goes, the studies would suggest somewhere between 15 and 30 milligrams in adults. So you titrate for kids, and how small they are, and starting a little lower and titrating up. With the liquid, with the capsules, our one capsule was, in our bioassay studies, is seeming to have some synergistic effect with PITC and coming in at about 15 milligrams. So I think Dr. McDonald is right on with the bigger kids being one, twice a day. Getting them close to a 30-milligram threshold, where it's having a significant effect on NRF2 and GGABAB.
David Roberts:
Great.
Martin:
Yeah.
David Roberts:
All right. Thank you for that. So, we are just about out of time. I do want to say thank you for everyone who's joined us. Dr. McDonald, thank you. I'm going to let you go, and we'll be in touch. But we do have a special through Friday, for those of you guys on the line who want to try it. That's an intro to professional strength, to this 15 milligrams in one capsule, six bottles for 270. So to get that, just email info@brocelite.com. Email your contact information, and we will call you and place that order. So, this price is additional 10% off wholesale price. Usually wholesale is 40% off, so this would be 50% off, if you're interested. We would even recommend, depending on your clinic, to buy more than six. But as far as people who want to dip their toe in before they dive in, this is the offer.
David Roberts:
And then we also are giving away one case, a year's supply of 12 bottles, to an individual. So, randomly selecting that individual is Clifton Thomas. Clifton, congratulations. If you could email info@brocelite.com your contact information and mailing information, and we'll get that out to you as soon as possible.
David Roberts:
All right, everyone. Thank you, Dr. Gildea. Thank you, Dr. Katz. We will be here next week as well, talking about the additional five other root causes as well as what science is saying for answers. Thank you so much.
Martin:
Good night. Thank you all.
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